Ion Exchange Chromatography of Glucagon in Urea-containing Buffers
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چکیده
منابع مشابه
Ion exchange chromatography of prolactin in urea-containing buffers.
Moore and Stein (1) have reviewed the work of Boardman and Partridge and others on problems encountered in attempting the chromatography of nonbasic proteins on Amberlite IRC-50 by elution analysis with the use of buffers of constant composition for the elution. Boardman and Partridge (2) suggested that the difficulty in achieving reversible equilibrium binding was due to hydrogen bonding betwe...
متن کاملIon exchange chromatography of glucagon in ureacontaining buffers.
Although the existence of the pancreatic hyperglycemic factor was postulated by Kimball and Murlin (1) in 1923, it was only recently that glucagon was isolated in crystalline form by Staub et al. (2). Glucagon was shown to be a basic polypeptide at that time. Such a peptide would be expected to yield successfully to chromatography on the carboxylic ion exchange resin Amberlite IRC-50, except fo...
متن کاملIon Exchange Chromatography
The separation and purification of various elements by ion exchange chromatography takes advantage of the variation of the electrostatic bond energies of ions in solution. Thus, the two main controlling factors in ion exchange chromatography are the ionic charge (z) and the ionic radius (r). These two are combined in the ionic potential – z/r – that can be used to compare relative electrostatic...
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Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that t...
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Shirai, Y. & Ohkubo, T. (1954a). J. Biochem., Tokyo, 41, 337. Shirai, Y. & Ohkubo, T. (1954b). J. Biochem., Tokyo, 41, 341. Storey, I. D. E. (1950). Biochem. J. 47, 212. Storey, I. D. E. & Dutton, G. J. (1955). Biochem. J. 59,279. Storey, I. D. E. & Dutton, G. J. (1956). Proc. 3rd Int. Congr. Biochem., Brussel8, 1955, p. 162. Strominger, J. L., Kalckar, H. M., Axelrod, J. & Maxwell, E. S. (1954...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64616-2